BE-FF identifies base editors to correct single nucleotide variations.

 

Taking under consideration the properties of various base editors (activity window, PAM etc.) BE-FF is able to detect precise corrections without bystander editing or synonymous corrections.

The ability of the tool to analyze the translation of a given sequence allows it to detect synonymous bystander editing.

Apparently, even some transversion mutations (i.e. purine -> pyrimidine and vice versa) can be corrected via base editing! Transversion corrections are made possible through base editing of bystander nucleotides and the reversal of the protein sequence.

The BE-FF DB presents human pathogenic SNPs that can be converted by base editing. You may also use the BE-FF online web tool to analyze your mutation of interest.

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If you find BE-FF helpful for your research, please cite:

Roy Rabinowitz, Shiran Abadi, Shiri Almog, Daniel Offen, Prediction of synonymous corrections by the BE-FF computational tool expands the targeting scope of base editing, Nucleic Acids Research, Volume 48, Issue W1, 02 July 2020, Pages W340–W347, https://doi.org/10.1093/nar/gkaa215