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CrisPam: SNP-derived PAM analysis tool for allele-specific targeting of genetic variants using CRISPR-Cas systems

The SNP-derived PAM approach allows precise allele-specific targeting.

The protospacer adjacent motif (PAM) is a short sequence, essential for both binding of the Cas to the DNA and the sequential DNA cleavage. 

Novel PAM sequences generated by a single-nucleotide variation (SNV) enable allele-specific binding and cleavage by the CRISPR-Cas system. 

Various CRISPR systems have different properties as well as different PAM sequences.

The diversity of Cas proteins yields a wide range of PAM sequences that can be utilized for SNP-derived PAM targeting. 

CrisPam identifies novel PAMs generated by a point mutation to design allele-specific gRNAs. 

Researchers can use CrisPam as an initial gRNA design tool to identify the suitable CRISPR system for their experiment. 


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