Offen’s Lab of Translational Neuroscience
Felsenstein Medical Research Center
Dept. of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine
Sagol School of Neuroscience
Tel Aviv University
CrisPam: SNP-derived PAM analysis tool for allele-specific targeting of genetic variants using CRISPR-Cas systems
The SNP-derived PAM approach allows precise allele-specific targeting.
The protospacer adjacent motif (PAM) is a short sequence, essential for both binding of the Cas to the DNA and the sequential DNA cleavage.
Novel PAM sequences generated by a single-nucleotide variation (SNV) enable allele-specific binding and cleavage by the CRISPR-Cas system.
Various CRISPR systems have different properties as well as different PAM sequences.
The diversity of Cas proteins yields a wide range of PAM sequences that can be utilized for SNP-derived PAM targeting.
CrisPam identifies novel PAMs generated by a point mutation to design allele-specific gRNAs.
Researchers can use CrisPam as an initial gRNA design tool to identify the suitable CRISPR system for their experiment.