Offen’s Lab of Translational Neuroscience
Felsenstein Medical Research Center
Dept. of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine
Sagol School of Neuroscience
Tel Aviv University
Web Tools by the Offen Group
SNP-derived PAM: Identify CRISPR systems to specifically target variant alleles .
Please view our CrisPam DB to find editable human pathogenic SNPs and the suitable CRISPR/Cas systems to perform allele-specific targeting.
You may also manually insert your sequence of interest.
Allele-specific targeting can be employed to target 90% of the SNPs!
BE-FF: Base Editors Functional Finder identifies base editors to correct SNVs. Taking under consideration the properties of various base editors (activity window, PAM etc.) BE-FF is able to detect precise corrections without bystander editing or synonymous corrections. The ability of the tool to analyze the translation of a given sequence yields better results.
Apparently, even some transversion mutations (i.e. purine -> pyrimidine and vice versa) are editable through base editing!
The BE-FF DB presents human pathogenic SNPs that can be precisely converted by base editing. You may also use the BE-FF online web tool to analyze your mutation of interest.
For more information please contact Roy Rabinowitz: rabinowitz.roy@gmail.com